| Name |
Description |
Order Applied |
| GSM5235776 channel 1 growth |
None |
1 |
| GSM5235776 channel 1 treatment |
Primed human pluripotent stem cells were maintained in StemFiT media (Ajinomoto) supplemented with 100 ng/ml bFGF on Laminin 511 E8 fragment-coated plates at 37 ºC in a humidified incubator with 20% O2 and 5% CO2. Naive human pluripotent stem cells were cultured in NDiff227 media (Takara) supplemented with 1 μM PD325901, 10 ng/ml of recombinant human leukemia inhibitory factor, 1 μM Gö6983 and 2 μM XAV939 on mitomycin C-treated MEF feeder. |
1 |
| GSM5235776 channel 1 extraction |
RNA was prepared using the miReasy Purification kit (Qiagen) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies). Illumina TruSeq Stranded Total RNA Library Prep |
1 |