| Name |
Description |
Order Applied |
| GSM2540094 channel 1 extraction |
Cells were lysed and total RNA isolated. RNA integrity was evaluated with a 2100 Bioanalyzer. Standard TruSeq protocols from Illumina. |
1 |
| GSM2540094 channel 1 growth |
Small molecule compounds were added to achieve a concentration corresponding to the ED20 and 1/10 of it, measured by cell viability at 60h after compound treatment. Vehicle control, including DMSO, ethanol and methanol were added in duplicate. |
1 |
| GSM2540094 channel 1 treatment |
H-STS cells were maintained in 1:1 mix of M199 : Ham's F-12 containing 10% FBS and antibiotics (penicillin 100 IU/ml and streptomycin 100 ug/ml). |
1 |