| Name |
Description |
Order Applied |
| GSM7245874 channel 1 growth |
Cells were transduced with non-targeting shRNA or one of three shEYA2 constructs. Cells are selected for with 1 ug/mL puromycin for 48 hours. The top 10% of zsGreen+ (included in shRNA constructs) weree sorted for. Cells were allowed to recover for 24 hours. RNA was extracted as described below. EYA2 KD was confirmed by qRT-PCR prior to RNA-Seq. |
1 |
| GSM7245874 channel 1 extraction |
RNeasy Plus Mini Kit (Qiagen 74136) Strand-specific poly(A)+-enriched mRNA (Illumina TruSEQ mRNA) |
1 |
| GSM7245874 channel 1 treatment |
Cells were maintained in DMEM supplemented with 10% FBS, 1% sodium pyruvate, 1% L-glutamate, 1% pen/strep |
1 |