| Name |
Description |
Order Applied |
| GSM777715 channel 1 treatment |
Freshly isolated CD4+CD25low/intCD127+ T cells; CD4+ T cells were cultured for 12 hrs followed by 8 hrs treatment as indicated. CD4+CD25+ Treg cells were expanded in cell culture. |
1 |
| GSM777715 channel 1 extraction |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific. |
1 |
| GSM777715 channel 1 growth |
Immediately after isolation or after described treatment, cells were lysed in Trizol reagent. |
1 |