| Name |
Description |
Order Applied |
| GSM430157 channel 1 labeling |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays |
1 |
| GSM430157 channel 1 extraction |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific. |
1 |
| GSM430157 channel 1 growth |
Immediately after isolation, cells were lysed in Trizol reagent or activated with CD3CD28 and either TGFb1 or PGE2 |
1 |
| GSM430157 channel 1 treatment |
Freshly isolated CD4+ T cells and CD4+ T cells separated into CD25 negativ and positiv subpopulation activated with variuos conditions |
1 |