| Name |
Description |
Order Applied |
| GSM430143 channel 1 extraction |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific. |
1 |
| GSM430143 channel 1 treatment |
freshly isolated CD4+ T cells were treated either immediately after isolation or after 18 hrs of cell culture with the indicated conditions |
1 |
| GSM430143 channel 1 growth |
immediately after isolation, cells were lysed in Trizol reagent. |
1 |
| GSM430143 channel 1 labeling |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays |
1 |