| Name |
Description |
Order Applied |
| GSM425335 channel 1 extraction |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific. |
1 |
| GSM425335 channel 1 labeling |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays |
1 |
| GSM425335 channel 1 growth |
immediately after isolation, cells were lysed in Trizol reagent or activated with CD3IL2 |
1 |
| GSM425335 channel 1 treatment |
freshly isolated CD4+ T cells, separated into CD25 negativ and positiv subpopulation |
1 |