| Name |
Description |
Order Applied |
| GSM422104 channel 1 labeling |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays |
1 |
| GSM422104 channel 1 extraction |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with NanoDrop ThermoScientific. |
1 |
| GSM422104 channel 1 growth |
Immediately after isolation, cells were lysed in Trizol reagent. Expanded Treg cells were CD3CD28 activated for a short time period and then lysed in Trizol reagent. |
1 |
| GSM422104 channel 1 treatment |
Freshly isolated CD4+ T cells as well as CD4+ T cells further separated into CD25 negativ and positiv subpopulation. Furthermore, CD4+CD25+ Treg cells were expanded in cell culture. |
1 |