Name |
Description |
Order Applied |
GSM143338 channel 1 labeling |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 micrograms of total RNA (Expression Analysis Technical Manual, Affymetrix). |
1 |
GSM143338 channel 1 treatment |
Iscove's modified Dulbecco’s medium supplemented with 10% FCS |
1 |
GSM143338 channel 1 extraction |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Gibco BRL). RNA was purified using the Rneasy Mini Kit according to the manufacturer's instruction (Qiagen). |
1 |