| Name |
Description |
Order Applied |
| GSM7869612 channel 1 growth |
Flp-In 293 T-REx cell lines stably expressing a CLIP-UPF1 mutant were reverse-transfected with 40 nM of the indicated siRNA using Lipofectamine RNAiMAX at a seed density of 300,000 in 6-well plates (without Hygromycin B), and RNA was harvested 72 hrs post-transfection. |
1 |
| GSM7869612 channel 1 extraction |
RNA was purified using the TRIzol Reagent (Invitrogen), treated with Shrimp dsDNase (Thermo Scientific), and cleaned up with RNAclean XP beads. 500 ng of total RNA was used per sample for library preparation. Ribosomal RNA was depleted using antisense rRNA oligonucleotides and thermostable RNase H, and libraries were generated using the xGen™ RNA Library Preparation Kit (IDT) |
1 |
| GSM7869612 channel 1 treatment |
Flp-In 293 T-REx cell lines were maintained in DMEM supplemented with 10% FBS , 100 U/ml penicillin, 100 μg/ml streptomycin , 0.3 mg/ml L-glutamine, and 100 µg/mL Hygromycin B (Invitrogen) at 37°C and 5% CO2. |
1 |