| GSM7998931 channel 1 extraction |
RNA was extracted using the Qiagen RNeasy Plus mini kit. Total RNA integrity was checked using an Agilent 4200 TapeStation (Agilent Technologies, Inc., Santa Clara, CA) and quantified using a Trinean DropSense96 spectrophotometer (Caliper Life Sciences, Hopkinton, MA). Samples with RIN < 5 were removed from further analysis. RNA was normalized to 50ng/ul and 500ng was submitted for library preparation. RNA-seq libraries were prepared from total RNA using the TruSeq Stranded mRNA kit (Illumina, Inc., San Diego, CA, USA). Sequencing was performed using an Illumina NovaSeq 6000 employing a paired-end, 50 base read length (PE50) sequencing strategy. RNA extraction was performed by the Specimen Processing & Research Cell Bank at Fred Hutch. Library preparation and sequencing was performed by the Genomics and Bioinformatics Core Services at Fred Hutch. |
1 |